Synaptobrevin 2, 1-89aa, Human, Recombinant, His- tagged

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01-2304
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Proteins, Recombinant
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Product Name Synaptobrevin 2, 1-89aa, Human, Recombinant, His- tagged
Background Synaptobrevin 2(Vehicle-associated membrane, VAMP2), which is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein (residues 1-89) forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25.
Synonyms VAMP2, SYB2, Synaptobrevin 2, VAMP-2, Synaptobrevin-2, Vesicle-associated membrane protein 2, vesicle-associated membrane protein 2 FLJ11460, RATVAMPB, RATVAMPIR, SYB, VAMP 2, Vesicle associated membrane protein 2, Vesicle-associated membrane protein 2 (synaptobrevin 2).
Species Human
Amino Acid Sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSA TAATAPPAAP AGEGGPPAPP PNLTSNRRLQ QTQAQVDEVV DIMRVNVDKV LERDQKLSEL DDRADALQAG ASQFETSAAKLKRKYW
Molecular Weight 11 kDa (108 aa), confirmed by MALDI-TOF.
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in Phosphate-Buffered Saline (pH 7.4)
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_055047
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Lin RC. and Scheller RH. (1997) Neuron. 19:1087-1094.
Hanson PI. et al (1997) Cell 90:523-535.
Scales SJ., et al. (2002) J Biol Chem. 277(31):28271-9.
Windoffer R et al. (1999) Cell Tissue Res 296:499-510

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