TBPL1, 1-186aa, Human

Proteins, Recombinant
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Product Name TBPL1, 1-186aa, Human
Background TBPL1, also known as STUD, TLF, TLP and TRF2, belongs to the TBP family. The TATA box-binding protein (TBP) is an essential component of the basal transcriptional machinery. Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. It is essential for spermiogenesis and believed to be important in expression of developmentally regulated genes. Recombinant human TBPL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms TATA box-binding protein-like protein 1, MGC8389, MGC9620, STUD, TLF, TLP, TRF2
Species Human
Molecular Weight 44.7 kDa (395aa), confirmed by MALDI-TOF
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 2mM DTT
Purity >90 % by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_004856
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Ohbayashi T., et al. (1999) Biochem. Biophys. Res. Commun. 255:137-142
Rabenstein M.D., et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96:4791-4796

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