| Product Name | TBPL1, 1-186aa, Human |
|---|---|
| Description | TBPL1, also known as STUD, TLF, TLP and TRF2, belongs to the TBP family. The TATA box-binding protein (TBP) is an essential component of the basal transcriptional machinery. Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. It is essential for spermiogenesis and believed to be important in expression of developmentally regulated genes. Recombinant human TBPL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | TATA box-binding protein-like protein 1, MGC8389, MGC9620, STUD, TLF, TLP, TRF2 |
| Host | E.coli |
| Molecular Weight | 44.7 kDa (395aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMDADSDV ALDILITNVV CVFRTRCHLN LRKIALEGAN VIYKRDVGKV LMKLRKPRIT ATIWSSGKII CTGATSEEEA KFGARRLARS LQKLGFQVIF TDFKVVNVLA VCNMPFEIRL PEFTKNNRPH ASYEPELHPA VCYRIKSLRA TLQIFSTGSI TVTGPNVKAV ATAVEQIYPF VFESRKEIL |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 2mM DTT |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | >90 % by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Ohbayashi T., et al. (1999) Biochem. Biophys. Res. Commun. 255:137-142 Rabenstein M.D., et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96:4791-4796 |
| Supplier | ARP |
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