THTPA, 1-230aa, Human

Category: Proteins
Catalog
01-1913
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Product Name THTPA, 1-230aa, Human
Description THTPA, also known as THTP or THTPASE, is a member of the THTPase family. This protein is localized to the cytoplasm and expressed at low levels in a variety of tissues, including testis, uterus, prostate, bladder, lung and kidney. THTPA is a hydrolase that catalyzes the H2O-dependent hydrolysis of thiamine triphosphate (THTP) to thiamine diphosphate (THDP), the major form of thiamine within the cell. THTPA exists as a monomer and functions at an optimal pH of 8.5. Recombinant human THTPA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms MGC2652, THTP, THTPASE, Thiamine-triphosphatase
Host E.coli
Molecular Weight 21.2kDa(188aa), confirmed by MALDI-TOF
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MAQGLIEVER KFLPGPGTEE RLQELGGTLE YRVTFRDTYY DTPELSLMQA DHWLRRREDS GWELKCPGAA GVLGPHTEYK ELTAEPTIVA QLCKVLRADG LGAGDVAAVL GPLGLQEVAS FVTKRSAWKL VLLGADEEEP QLRVDLDTAD FGYAVGEVEA LVHEEAEVPT ALEKIHRLSS MLGVPAQETA PAKLIVYLQR FRPQDYQRLL EVNSSRERPQ ETEDPDHCLG
Tag His-tag
Reactivity Human
Applications SDS-PAGE
Form Liquid, in 20 mM Tris-HCl Buffer (pH 8.0) containing 100 mM NaCl, 0.1 mM PMSF, 10% Glycerol
Concentration 0.5 mg/ml (determined by Bradford assay)
Purity > 90 % by SDS-PAGE
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
References Lakaye B., et al. (2002) J Biol Chem. 277(16):13771-7.
Lakaye B., et al. (2004) Int J Biochem Cell Biol. 36(7):1348-64.
Supplier ARP

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