TRXB, 1-321aa

Catalog
01-1822
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Proteins, Recombinant
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Product Name TRXB, 1-321aa
Background TRXB(Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E.coli TRXB protein was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms , Thioredoxin reductase JW0871, ECK0879, Thioredoxin reductase, FAD/NAD(P)-binding, TRXR.
Species E. coli
Amino Acid Sequence MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K
Molecular Weight 17.2 kDa (160aa), confirmed by MALDI-TOF
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT.
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # YP_003044110
Host E.coli
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Faulkner MJ., et al. (2008) Proc Natl Acad Sci U S A. 105(18):6735-40.
Takahata M., et al. (2008) J Biochem. 143(4):467-73.

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