| Product Name | TXN, 1-105aa, Human, Recombinant |
|---|---|
| Description | SQSTM1 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1 and play a role in titin/TTN downstream signaling in muscle cells. This protein also may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of the sequestosome 1 protein commonly cause Paget's disease since the UBA is necessary for aggregate sequestration and cell survival. Recombinant SQSTM1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | TRX, TRX1, Thioredoxin JW0871, ECK0879, Thioredoxin reductase, FAD/NAD(P)-binding, TRXR. |
| Host | E.coli |
| Molecular Weight | 39.8 kDa (343aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 2mM EDTA, 0.1M NaCl |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Pigiet VP., et al. (1986) Proc Natl Acad Sci USA. 83(20):7643-7. Lundstrom J., et al. (1990) J Biol Chem. 265(16):9114-20. |
| Supplier | ARP |
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