UBE2D4, 1-147aa, Human

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01-2014-D4
$0.00
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Proteins, Recombinant
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Product Name UBE2D4, 1-147aa, Human
Background UBE2D4 belongs to the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Recombinant human UBE2D4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Ubiquitin-conjugating enzyme E2 D4, UBCH5D, HBUCE1
Species Human
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV PEIAHTYKAD REKYNRLARE WTQKYAM
Molecular Weight 24.4 kDa (227aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Concentration 1 mg/ml (determined by Bradford)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_057067
Host E.coli
Tag His-tag
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References David Y., et al. (2010) J. Biol. Chem. 285:8595-8604
Nandi, D, et al. (2006) Journal of biosciences 31 (1): 137-55.

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