| Product Name | UBE2T, 1-197aa, Human, Recombinant |
|---|---|
| Description | UBE2T, also known as PIG50 or HSPC150, is a member of the E2 ubiquitin-conjugating enzyme family Involved in the protein degradation pathway. This protein catalyzes the ATP-dependent attachment of ubiquitin (Ub) to target proteins, thereby tagging them for subsequent destruction by the proteasome. Additionally, UBE2T is thought to be a crucial component of the Faconi anemia pathway of DNA damage repair and, upon self-inactivation, may negatively regulate the Faconi pathway. Recombinant UBE2T protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | HSPC150, PIG50, Ubiquitin-conjugating enzyme E2T Cell proliferation inducing gene 50 protein, HSPC150 protein similar to ubiquitin conjugating enzyme, HSPC150 protein similar to ubiquitin-conjugating enzyme, UBE2T, Ubiquitin carrier protein T, Ubiquitin conjugating enzyme, Ubiquitin conjugating enzyme E2T, Ubiquitin protein ligase T, |
| Host | E.coli |
| Molecular Weight | 10.8 Da (93aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDVLEH HHHHH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Hao J., et al. (2008) Tumour Biol. 29(3):195-203. Machida YJ., et al. (2006) Mol Cell. 23(4):589-96. |
| Supplier | ARP |
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