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|Product Name||USF1, 1-310aa, Human|
|Background||USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. USF1 can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21. Recombinant human USF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MGSMKGQQKT AETEEGTVQI QEGAVATGED PTSVAIASIQ SAATFPDPNV KYVFRTENGG QVMYRVIQVS
EGQLDGQTEG TGAISGYPAT QSMTQAVIQG AFTSDDAVDT EGTAAETHYT YFPSTAVGDG AGGTTSGSTA AVVTTQGSEA LLGQATPPGT
GQFFVMMSPQ EVLQGGSQRS IAPRTHPYSP KSEAPRTTRD EKRRAQHNEV ERRRRDKINN WIVQLSKIIP DCSMESTKSG QSKGGILSKA
CDYIQELRQS NHRLSEELQG LDQLQLDNDV LRQQVEDLKN KNLLLRAQLR HHGLEVVIKN DSN
|Molecular Weight||36.9 kDa(333aa) confirmed by MALDI-TOF|
|Concentration||0.5 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
0.15M NaCl,1mM DTT, 0.1mM PMSF
|Purity||> 90% by SDS-PAGE|
|NCBI Accession #||NP_001263302|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Rahaus M., et al. (2003) J. Gen. Virol. 84:2957-2967
Pajukanta P., et al. (2004) Nat. Genet. 36:371-376
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