| Product Name | WBP2, 1-261aa, Human |
|---|---|
| Description | WBP2 (WW domain-binding protein 2) is a steroid hormone receptor coactivator. This protein contains 1 GRAM domain. The globular WW domain is composed of 38 to 40 semiconserved amino acids shared by proteins of diverse functions including structural, regulatory, and signaling proteins. The domain is involved in mediating protein-protein interactions through the binding of polyproline ligands. It binds to the WW domain of Yes kinase-associated protein(YAP1) by its PY motifs. The WW-binding 1 motif mediates interaction with NEDD4. Recombinant human WBP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | WW domain binding protein 2, WBP-2 |
| Host | E.coli |
| Molecular Weight | 43.7 kDa (374aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMALNKNH SEGGGVIVNN TESILMSYDH VELTFNDMKN VPEAFKGTKK GTVYLTPYRV IFLSKGKDAM QSFMMPFYLM KDCEIKQPVF GANYIKGTVK AEAGGGWEGS ASYKLTFTAG GAIEFGQRML QVASQASRGE VPSGAYGYSY MPSGAYVYPP PVANGMYPCP PGYPYPPPPP EFYPGPPMMD GAMGYVQPPP PPYPGPMEPP VSGPDVPSTP AAEAKAAEAA ASAYYNPGNP HNVYMPTSQP PPPPYYPPED KKTQ |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol,1mM DTT |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Chen H.I., et al. (1997) J. Biol. Chem. 272:17070-17077 Pirozzi G., et al. (1997) J. Biol. Chem. 272:14611-14616 |
| Supplier | ARP |
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