WIBG, 1-204aa, Human

Catalog
01-1886
$0.00
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Proteins, Recombinant
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Product Name WIBG, 1-204aa, Human
Background WIBG has been identified as an interacting partner of Mago-Y14. The Mago-Y14 heterodimer is a core component of the EJC(exon junction complex) that is deposited on mRNAs as a consequence of splicing and influences postsplicing mRNA metabolism. This protein is a cytoplasmic RNA-binding protein that is excluded from the nucleus by Crm1. It interacts directly with Mago-Y14 by means of its N-terminal domain. Recombinant human WIBG protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms PYM, Partner of Y14 and mago
Species Human
Amino Acid Sequence MEAAGSPAAT ETGKYIASTQ RPDGTWRKQR RVKEGYVPQE EVPVYENKYV KFFKSKPELP PGLSPEATAP VTPSRPEGGE PGLSKTAKRN LKRKEKRRQQ QEKGEAEALS RTLDKVSLEE TAQLPSAPQG SRAAPTAASD QPDSAATTEK AKKIKNLKKK LRQVEELQQR IQAGEVSQPS KEQLEKLARR RALEEELEDL ELGLLEHHHH HH
Molecular Weight 23.3 kDa (210aa), confirmed by MALDI-TOF
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl
Purity > 85% by SDS-PAGE
Applications SDS-PAGE
NCBI Accession # NP_115721
Host E.coli
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Bono F,. et al. (2004) EMBO Rep. 5(3):304-10.
Gehring NH,,et al. (2009) Cell. 137(3):536-48.

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