Product Name | XRCC3, 1-346aa, Human |
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Description | XRCC3, also known as RAD51-like, belongs to the RecA family and RAD51 subfamily. It participates in homologous recombination to maintain chromosome stability and repair DNA damage. This protein functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. Recombinant human XRCC3 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
Synonyms | X-ray repair cross complementing protein 3, RAD51-like |
Host | E.coli |
Molecular Weight | 30.9 kDa (281aa), confirmed by MALDI-TOF |
Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MDLDLLDLNP RIIAAIKKAK LKSVKEVLHF SGPDLKRLTN LSSPEVWHLL RTASLHLRGS SILTALQLHQ QKERFPTQHQ RLSLGCPVLD ALLRGGLPLD GITELAGRSS AGKTQLALQL CLAVQFPRQH GGLEAGAVYI CTEDAFPHKR LQQLMAQQPR LRTDVPGELL QKLRFGSQIF IEHVADVDTL LECVNKKVPV LLSRGMARLV VIDSVAAPFR CEFDSQASAP RARHLQSLGA TLRELSSAFQ SPVLCINQVT EAMEEQGAAH GPLGFWDERV SPALGITWAN QLLVRLLADR LREEEAALGC PARTLRVLSA PHLPPSSCSY TISAEGVRGT PGTQSH |
Tag | His-tag |
Reactivity | Human |
Applications | SDS-PAGE |
Form | Liquid, in 20mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 50% glycerol, 2mM DTT |
Concentration | 0.25 mg/ml (determined by Bradford assay) |
Purity | > 85% by SDS-PAGE |
Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
References |
Tebbs RS. et al. (1995) Proc Natl Acad Sci U S A. 92:6354-6358. Miller KA. et al. (2002) J Biol Chem. 277:8406-8411. |
Supplier | ARP |
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