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|Product Name||TAGLN2, 13-199aa, Human|
|Background||TAGLN2, also known transgelin 2, is one of the earliest markers of differentiated smooth muscle. This protein contains a calponin like repeat and a calponin-homology (CH) domain. It is downregulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. It also binds actin, causing actin fibers to gel within minutes of binding. Binding of transgelin to actin occurs at a ratio of 1:6 actin monomers. Recombinant human TAGLN2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
|Synonyms||Transgelin-2, HA1756, KIAA0120, Transgelin 2|
|AA Sequence||MGSSHHHHHH SSGLVPRGSH MEVQQKIEKQ YDADLEQILI QWITTQCRKD VGRPQPGREN FQNWLKDGTV LCELINALYP EGQAPVKKIQ ASTMAFKQME QISQFLQAAE RYGINTTDIF QTVDLWEGKN MACVQRTLMN LGGLAVARDD GLFSGDPNWF PKKSKENPRN FSDNQLQEGK NVIGLQMGTN RGASQAGMTG YGMPRQIL|
|Molecular Weight||24.9 kDa (222aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT|
|Concentration||1.0 mg/ml (determined by BCA assay)|
|Purity||> 90% by SDS-PAGE|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Shapland C, et al. (1993). J. Cell Biol., 121:1065-1073.
Kobayashi R, et al. (1994). Biochem. Biophys. Res. Commun. 198:1275-1280.
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