TAGLN2, 13-199aa, Human

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Product Name TAGLN2, 13-199aa, Human
Background TAGLN2, also known transgelin 2, is one of the earliest markers of differentiated smooth muscle. This protein contains a calponin like repeat and a calponin-homology (CH) domain. It is downregulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. It also binds actin, causing actin fibers to gel within minutes of binding. Binding of transgelin to actin occurs at a ratio of 1:6 actin monomers. Recombinant human TAGLN2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Species Human
Synonyms Transgelin-2, HA1756, KIAA0120, Transgelin 2
Host E.coli
Tag His-tag
Molecular Weight 24.9 kDa (222aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT
Concentration 1.0 mg/ml (determined by BCA assay)
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI NP_003555
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Shapland C, et al. (1993). J. Cell Biol., 121:1065-1073.
Kobayashi R, et al. (1994). Biochem. Biophys. Res. Commun. 198:1275-1280.

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