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|Product Name||TIGAR, 1-270aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MGSHMARFAL TVVRHGETRF NKEKIIQGQG VDEPLSETGF KQAAAAGIFL NNVKFTHAFS SDLMRTKQTM HGILERSKFC KDMTVKYDSR LRERKYGVVE GKALSELRAM AKAAREECPV FTPPGGETLD QVKMRGIDFF EFLCQLILKE ADQKEQFSQG SPSNCLETSL AEIFPLGKNH SSKVNSDSGI PGLAASVLVV SHGAYMRSLF DYFLTDLKCS LPATLSRSEL MSVTPNTGMS LFIINFEEGR EVKPTVQCIC MNLQDHLNGL TETR|
|Background||TIGAR, also known as TP53-induced glycolysis and apoptosis regulator, is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. These facts indicate that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses. Recombinant human TIGAR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Concentration||1mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris buffer (pH 8.0) containing 10% glycerol, 1mM DTT|
|Intended Use||For Research Use Only|
|Molecular Weight||14.9 kDa (137aa), confirmed by MALDI-TOF|
|NCBI Accession #||NP_065108|
|Purity||> 90% by SDS-PAGE|
|References||Bensaad K., et al. (2009) EMBO J. 28(19):3015-26.
Hasegawa H., et al. (2009) Leukemia. 23(11):2090-101.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||TP53-induced glycolysis and apoptosis regulator,|
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