TUBG1, 1-451aa, Human

Category: Proteins
Catalog
01-P1295
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Product Name TUBG1, 1-451aa, Human
Description TUBG1 is a member of the tubulin superfamily. This protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. It mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. Recombinant human TUBG1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
Synonyms Tubulin gamma-1 chain, GCP-1, TUBG, TUBGCP1
Host E.coli
Molecular Weight 28.5 kDa (254aa), confirmed by MALDI-TOF
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MPREIITLQL GQCGNQIGFE FWKQLCAEHG ISPEGIVEEF ATEGTDRKDV FFYQADDEHY IPRAVLLDLE PRVIHSILNS PYAKLYNPEN IYLSEHGGGA GNNWASGFSQ GEKIHEDIFD IIDREADGSD SLEGFVLCHS IAGGTGSGLG SYLLERLNDR YPKKLVQTYS VFPNQDEMSD VVVQPYNSLL TLKRLTQNAD CVVVLDNTAL NRIATDRLHI QNPSFSQINQ LVSTIMSAST TTLRYPGYMN NDLIGLIASL IPTPRLHFLM TGYTPLTTDQ SVASVRKTTV LDVMRRLLQP KNVMVSTGRD RQTNHCYIAI LNIIQGEVDP TQVHKSLQRI RERKLANFIP WGPASIQVAL SRKSPYLPSA HRVSGLMMAN HTSISSLFER TCRQYDKLRK REAFLEQFRK EDMFKDNFDE MDTSREIVQQ LIDEYHAATR PDYISWGTQE Q
Tag His-tag
Reactivity Human
Applications SDS-PAGE
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 1mM DTT, 0.1mM PMSF
Concentration 0.5 mg/ml (determined by Bradford assay)
Purity > 85 % by SDS-PAGE
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
References Zheng Y., et al. (1991) Cell. 65(5):817-23.
Wise DO., et al. (2000) Genomics. 67(2):164-70.
Supplier ARP

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