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|Product Name||UBE2D4, 1-147aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV PEIAHTYKAD REKYNRLARE WTQKYAM|
|Background||UBE2D4 belongs to the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Recombinant human UBE2D4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Concentration||1 mg/ml (determined by Bradford)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT|
|Intended Use||For Research Use Only|
|Molecular Weight||24.4 kDa (227aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)|
|NCBI Accession #||NP_057067|
|Purity||> 95% by SDS-PAGE|
|References||David Y., et al. (2010) J. Biol. Chem. 285:8595-8604
Nandi, D, et al. (2006) Journal of biosciences 31 (1): 137-55.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||Ubiquitin-conjugating enzyme E2 D4, UBCH5D, HBUCE1|
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