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|Product Name||UBE2J2, 1-226aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MGSHMSSTSS KRAPTTATQR LKQDYLRIKK DPVPYICAEP LPSNILEWHY VVRGPEMTPY EGGYYHGKLI FPREFPFKPP SIYMITPNGR FKCNTRLCLS ITDFHPDTWN PAWSVSTILT GLLSFMVEKG PTLGSIETSD FTKRQLAVQS LAFNLKDKVF CELFPEVVEE IKQKQKAQDE LSSRPQTLPL PDVVPDGETH LVQNGIQLLN GHAPGAVPNL AGLQQANRHH|
|Background||UBE2J2, also known as ubiquitin-conjugating enzyme E2 J2, belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Recombinant human UBE2J2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.2M NaCl, 1mM DTT|
|Intended Use||For Research Use Only|
|Molecular Weight||23.5 kDa (207aa) confirmed by MALDI-TOF|
|NCBI Accession #||NP_477515|
|Purity||> 90% by SDS-PAGE|
|References||Wang X, et al. (2009) J Cell Biol. 187(5):655-68.
Nandi, D. et al. (2006) Journal of biosciences 31 (1): 137-55.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||ubiquitin-conjugating enzyme E2 J2, NCUBE-2, NCUBE2, PRO2121|
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