1,5-AG ELISA kit (General species)

Category: Assay Kits
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MEB046Ge
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Product Name 1,5-AG ELISA kit (General species)
Synonyms LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1,5-Anhydro-D-glucitol; 1,5-Anhydrosorbitol; Aceritol; Polygalytol
Method Competitive
Detection Range 1.9-150ug/mL
Sensitivity 0.8ug/mL
Target 1,5-AG
Specificity This assay has high sensitivity and excellent specificity for detection of Mini Samples 1,5-Anhydroglucitol (1,5-AG). No significant cross-reactivity or interference between Mini Samples 1,5-Anhydroglucitol (1,5-AG) and analogues was observed.
Sample Types Serum, Plasma And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 25uL standard or sample to each well. And then add 25μL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 50uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 50uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 25uL Stop Solution.Read at 450 nm immediately.
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
Supplier Cloud Clone

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