ELISA

ELISA

Enzyme-linked immunosorbent assay (ELISA) is an established laboratory technique to detect and quantify a target molecule within a sample. The basic ELISA principle involves the use of an enzyme-conjugated antibody to detect an antigen which has been immobilized to a solid matrix, typically the wells of a microtiter plate.

What are the different types of ELISA?

Several types of ELISA have been developed since the technique was first described. In a direct ELISA, the antigen is adsorbed to the microtiter plate and is detected using an enzyme-conjugated primary antibody. The indirect ELISA technique requires an additional protocol step since an enzyme-conjugated secondary antibody is used for detection. During a sandwich ELISA, the antigen is captured between two antigen-specific antibodies, one of which is enzyme-conjugated and the other adsorbed to the microtiter plate. In each method, the amount of antigen present in the sample is usually determined by enzyme-mediated catalysis of a colorimetric substrate.

Ready-to-use commercial kits

Variations of all three basic ELISA methods have been developed to increase assay sensitivity and throughput, and a wide range of ready-to-use ELISA kits is commercially available. Containing all the necessary components to detect and measure a specific analyte, pre-configured ELISA kits deliver considerable time savings by reducing the need for in-house assay development and optimization.
American Research Products supplies an extensive range of ELISA kits that are designed to quantitatively measure specific antigens with a high degree of sensitivity.

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  1. 11-Dehydrothromboxane B2 (11DHTXB2) ELISA Kit
    11-Dehydrothromboxane B2 (11DHTXB2) ELISA Kit
    UNEB0058
    15.6-1000 pg/mL
    General
  2. 12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit (UNES00058)
    12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit (UNES00058)
    UNES00058
    0.63ng/mL
    0.78--50ng/mL
    Universal
  3. 12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit
    12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit
    E-EL-0109
    0.63ng/mL
    0.78-50ng/mL
    General
  4. 12-Hydroxyeicosatetraenoic acid (12HETE) ELISA Kit
    12-Hydroxyeicosatetraenoic acid (12HETE) ELISA Kit
    UNEB0059
    0.156-10 ng/mL
    General
  5. 15d-PGJ2(15-Deoxy-Delta12,14-prostaglandin J2)ELISA
    15d-PGJ2 (15-Deoxy-Delta12,14-prostaglandin J2)ELISA Kit (UNES00055)
    UNES00055
    1.875ng/mL
    3.125--200ng/mL
    Universal
  6. 15d-PGJ2(15-Deoxy-Delta12,14-prostaglandin J2) ELISAÊKit
    15d-PGJ2(15-Deoxy-Delta12,14-prostaglandin J2) ELISAÊKit
    E-EL-0087
    1.875ng/mL
    3.125-200ng/mL
    General
  7. 17-Hydroxyprogesterone (17OHP) ELISA Kit
    17-Hydroxyprogesterone (17OHP) ELISA Kit
    UNEB0005
    0.312-20 ng/mL
    General
  8. 2,3-Bisphosphoglycerate (2,3-BPG) ELISA Kit
    2,3-Bisphosphoglycerate (2,3-BPG) ELISA Kit
    UNEB0057
    31.2-2000 nmol/mL
    General
  9. 2,3-dinor-TXB2 / 2,3-dinor Thromboxane B2  ELISA Kit
    2,3-dinor-TXB2 / 2,3-dinor Thromboxane B2 ELISA Kit
    UNFI0046
    9.375 pg/ml
    15.625-1000pg/ml
    General
  10. 2-Methoxy Estradiol ELISA Kit
    2-Methoxy Estradiol ELISA Kit
    UNFI0017
    31 ng/ml
    61.25-2000ng/ml
    General

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