ELISA

ELISA

Enzyme-linked immunosorbent assay (ELISA) is an established laboratory technique to detect and quantify a target molecule within a sample. The basic ELISA principle involves the use of an enzyme-conjugated antibody to detect an antigen which has been immobilized to a solid matrix, typically the wells of a microtiter plate.

What are the different types of ELISA?

Several types of ELISA have been developed since the technique was first described. In a direct ELISA, the antigen is adsorbed to the microtiter plate and is detected using an enzyme-conjugated primary antibody. The indirect ELISA technique requires an additional protocol step since an enzyme-conjugated secondary antibody is used for detection. During a sandwich ELISA, the antigen is captured between two antigen-specific antibodies, one of which is enzyme-conjugated and the other adsorbed to the microtiter plate. In each method, the amount of antigen present in the sample is usually determined by enzyme-mediated catalysis of a colorimetric substrate.

Ready-to-use commercial kits

Variations of all three basic ELISA methods have been developed to increase assay sensitivity and throughput, and a wide range of ready-to-use ELISA kits is commercially available. Containing all the necessary components to detect and measure a specific analyte, pre-configured ELISA kits deliver considerable time savings by reducing the need for in-house assay development and optimization.
American Research Products supplies an extensive range of ELISA kits that are designed to quantitatively measure specific antigens with a high degree of sensitivity.

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  1. 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit
    1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit
    abx350730
    7.813 pg/ml - 500 pg/ml
    General
  2. 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit
    1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit
    abx156662
    123.5 pg/ml - 10000 pg/ml
    General
  3. 1,5-Anhydroglucitol (1,5-AG) ELISA Kit
    1,5-Anhydroglucitol (1,5-AG) ELISA Kit
    abx150305
    1.9 _g/ml - 150 _g/ml
    General
  4. 1-Desamino 8D Arginine Vasopressin (DDAVP) ELISA Kit
    1-Desamino 8D Arginine Vasopressin (DDAVP) ELISA Kit
    abx150333
    123.46 pg/ml - 10000 pg/ml
    General
  5. 11,12-Dihydroxyeicosatrienoic Acids (11,12-DiHETrE) ELISA Kit
    11,12-Dihydroxyeicosatrienoic Acids (11,12-DiHETrE) ELISA Kit
    abx352136
    1.56 ng/ml - 100 ng/ml
    General
  6. 11-Dehydrothromboxane B2 (11-Dehydro-TXB2) ELISA Kit
    11-Dehydrothromboxane B2 (11-Dehydro-TXB2) ELISA Kit
    abx514539
    15.6 pg/ml - 1000 pg/ml
    General
  7. 11-Dehydrothromboxane B2 (11DHTXB2) ELISA Kit
    11-Dehydrothromboxane B2 (11DHTXB2) ELISA Kit
    UNEB0058
    15.6-1000 pg/mL
    General
  8. 11-Dehydrothromboxane B3 (11-Dehydro-TXB2) ELISA Kit
    11-Dehydrothromboxane B3 (11-Dehydro-TXB2) ELISA Kit
    abx251255
    15.625 pg/ml - 1000 pg/ml
    General
  9. 12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit (UNES00058)
    12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit (UNES00058)
    UNES00058
    0.63ng/mL
    0.78--50ng/mL
    Universal
  10. 12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit
    12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit
    E-EL-0109
    0.63ng/mL
    0.78-50ng/mL
    General

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