Monoclonal antibodies (MAbs) are valuable tools for biological research, diagnostic immunoassays and therapeutic purposes. In the research field, MAbs are used in a wide variety of techniques including popular applications such Flow cytometry, western blotting, Enzyme-linked immunosorbent assays (ELISA) and immunohistology. Since Kohler & Milstein developed hybridoma technology in 1975, tens of thousands of monoclonal antibodies have been developed and manufactured against a vast array of biological targets. The successful use of monoclonal antibodies in biological research is primarily due to the specificity and homogeneity of antibody produced by the hybridoma and the potential to be able to produce unlimited amounts a specific Mab.
ARP American Research Products provides an extensive range of monoclonal antibodies. The product range includes high-quality antibodies, designed for research use, against viral antigens and CD markers that are of potential diagnostic and therapeutic interest.
Monoclonal antibodies recognising Adeno-associated virus (AAV)
Adeno-associated viruses A(AAV), from the parvovirus family, are small single-stranded DNA viruses that affect humans and some additional primate species. AAV is currently considered to be non-pathogenic and most people infected with AAV only develop a mild immune response. Adeno-associated viruses carry genes into both dividing and quiescent cells where they can insert genetic information into the host genome These characteristics of AAV have been exploited for gene therapy and AAV virus have been engineered to carry therapeutic genes into host cells. A range of monoclonal antibodies is available from ARP which recognize intact AAV particles and capsid proteins VP1, VP2 and VP3.
- 03-61058 Anti-Adeno-associated virus (AAV), VP1/VP2/VP 3
- 03-65158 Anti-Adeno-associated virus (AAV), VP1/VP2/VP3
- 03-61055 Anti-Adeno-associated virus (AAV), intact particles
- 03-610148 Anti-Adeno-associated virus type 5 (AAV-5), intact particles
ARP also supply a mouse monoclonal antibody against rabies virus (12-5130-2), which is conjugated to the FITC fluorochrome. This antibody is suitable for use in a range of applications including immunofluorescence, ELISA, immunohistochemistry and immunoblotting.
Erythrocyte marker CD233
Mouse monoclonal antibody (clone BRIC6) recognises human CD233, also known as SLC4A1, which is an integral membrane protein in human erythrocytes. CD233 associates with the erythrocyte membrane protein glycophorin A (GPA) which promotes the correct folding and translocation during biosynthesis of CD23. Many CD233 mutations are known to man which give rise to blood group antigens that form the Diego blood group system. Gene mutations can also lead to destabilization of the erythrocyte membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. The CD233 antibody (08-9439-4) is conjugated to the fluorochrome phycoerythrin (PE) and is suitable for flow cytometry applications.
Affinity purified mouse monoclonal antibody recognising human CD59
CD59 is a cell surface glycoprotein that regulates complement-mediated cell lysis and is involved in lymphocyte signal transduction. CD59 is broadly distributed among hematopoietic and non - hematopoietic cells such as B-cells, T-cells, monocytes, epithelium, platelets, polymorphonuclear neutrophils and endothelium. Patients with the disease paroxysmal nocturnal hemoglobinuria (PNH) show a reduced cellular expression of CD59. Recent reports suggest that CD59 overexpression may be a biomarker of poor prognosis in breast cancer. ARP supply an affinity purified monoclonal antibody (BRIC229), which recognises human CD59 that is suitable for immunoblotting and neutralization studies (08-9409-2).