15-HETE ELISA Kit

Category: Assay Kits
Catalog
A303889
( Ships in 5-10 business days )

Questions? Contact us

Call (800) 832-2611

arp-guarantee
- +
$0.00
More Information
Product Name 15-HETE ELISA Kit
Description 15-HETE ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of 15-HETE in serum, plasma, tissue homogenates, and other biological fluids.
Method Competitive
Detection Range 0.156-10 ng/ml
Sensitivity 0.094 ng/ml
Target 15-HETE
Reactivity All
Applications ELISA
Sample Types Serum, plasma, tissue homogenates, and other biological fluids.
Reconstitution 15-HETE ELISA Kit (A303889) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal 15-HETE in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with 15-HETE antigen. During the incubation, 15-HETE present in the samples or standards competes with the fixed amount of immobilized 15-HETE for binding sites on the Biotinylated Anti-15-HETE Antibody. The more 15-HETE present in a sample or standard, the less Biotinylated Anti-15-HETE Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-15-HETE Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of 15-HETE present in each sample or standard. The concentration of 15-HETE can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Storage Store at +4°C. Do not use past expiration date!
Supplier Antibodies.com

All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.