15-LO-2 ELISA kit (Human)

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Product Name 15-LO-2 ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Arachidonate-15-Lipoxygenase, Type B (ALOX15B). No significant cross-reactivity or interference between Arachidonate-15-Lipoxygenase, Type B (ALOX15B) and analogues was observed.
Synonyms ALOX15B; LOX15B; 15-LOX-2; Arachidonate 15-lipoxygenase type II; Lipoxygenase 15, Type B; 15-lipoxygenase 2; Linoleate 13-lipoxygenase 15-LOb Arachidonate-15-Lipoxygenase, Type B
Method Sandwich
Detection Range 0.156-10ng/mL
Sensitivity 0.059ng/mL
Target 15-LO-2
Specificity This assay has high sensitivity and excellent specificity for detection of 15-Lipoxygenase-2 (15-LO-2). No significant cross-reactivity or interference between 15-Lipoxygenase-2 (15-LO-2) and analogues was observed.
Reactivity Human
Sample Types Serum, Plasma, Tissue Homogenates And Other Biological Fluids.
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot O15296
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
Supplier Cloud Clone


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