15d-PGJ2(15-Deoxy-Delta12,14-prostaglandin J2) ELISA Kit

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Product Name 15d-PGJ2(15-Deoxy-Delta12,14-prostaglandin J2) ELISA Kit
Description This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 15d-PGJ2. During the reaction, 15d-PGJ2 in the sample or standard competes with a fixed amount of 15d-PGJ2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 15d-PGJ2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 15d-PGJ2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Synonyms 15-Deoxy-Delta12, 14-prostaglandin J2
Method Competitive ELISA
Detection Range 3.13~200ng/mL
Sensitivity 1.88ng/mL
Target 15d-PGJ2
Specificity This kit recognizes 15d-PGJ2 in samples. No significant cross-reactivity or interference between 15d-PGJ2 and analogues was observed.
Sample Types Serum, plasma and other biological fluids
Reconstitution This ELISA kit applies to the in vitro quantitative determination of 15d-PGJ2 concentrations in Serum, plasma and other biological fluids.
Storage An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
Supplier Elabscience


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