17-OHP ELISA kit (General species)

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CEA454Ge
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Product Name 17-OHP ELISA kit (General species)
Description This assay has high sensitivity and excellent specificity for detection of 17-Hydroxyprogesterone (17-OHP). No significant cross-reactivity or interference between 17-Hydroxyprogesterone (17-OHP) and analogues was observed.
Synonyms 17OHP; 17-OH Progesterone; 17-Alpha-Hydroxyprogesterone
Method Competitive
Detection Range 246.9-20,000pg/mL
Sensitivity 87.3pg/mL
Target 17-OHP
Specificity This assay has high sensitivity and excellent specificity for detection of 17-Hydroxyprogesterone (17-OHP). No significant cross-reactivity or interference between 17-Hydroxyprogesterone (17-OHP) and analogues was observed.
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately.
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
Supplier Cloud Clone

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