| Product Name | 3-Methoxy-4-hydroxyphenylglycol ELISA Kit |
|---|---|
| Description | 3-Methoxy-4-hydroxyphenylglycol ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of 3-Methoxy-4-hydroxyphenylglycol in serum, plasma, tissue homogenates, and other biological fluids. |
| Method | Competitive |
| Detection Range | 0.156-10 ng/ml |
| Sensitivity | 0.094 ng/ml |
| Target | 3-Methoxy-4-hydroxyphenylglycol |
| Reactivity | All |
| Applications | ELISA |
| Sample Types | Serum, plasma, tissue homogenates, and other biological fluids. |
| Reconstitution | 3-Methoxy-4-hydroxyphenylglycol ELISA Kit (A247120) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal 3-Methoxy-4-hydroxyphenylglycol in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with 3-Methoxy-4-hydroxyphenylglycol antigen. During the incubation, 3-Methoxy-4-hydroxyphenylglycol present in the samples or standards competes with the fixed amount of immobilized 3-Methoxy-4-hydroxyphenylglycol for binding sites on the Biotinylated Anti-3-Methoxy-4-hydroxyphenylglycol Antibody. The more 3-Methoxy-4-hydroxyphenylglycol present in a sample or standard, the less Biotinylated Anti-3-Methoxy-4-hydroxyphenylglycol Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-3-Methoxy-4-hydroxyphenylglycol Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of 3-Methoxy-4-hydroxyphenylglycol present in each sample or standard. The concentration of 3-Methoxy-4-hydroxyphenylglycol can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve. |
| Storage | Store at +4°C. Do not use past expiration date! |
| Supplier | Antibodies.com |
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