| Product Name | 5-FAM-LPETGG |
|---|---|
| Description | 5-FAM-LPETGG is the sortase recognition sequence, LPETGG, coupled to the fluorescent label 5-FAM. 5-FAM-LPETGG can be used for site-specific labeling of target proteins using sortase-catalyzed transpeptidation. In this process, sortase A cleaves between the thr and gly residues, forming a thioester intermediate with the peptide probe. A protein N-terminally engineered with a series of glycine residues resolves this intermediate, regenerating the active site cysteine of sortase and conjugating the probe to the N-terminus of the protein. 5-FAM-LPETGG contains the a 5- carboxyfluorescein label, which is one of the most widely used green fluorescent reagents for in situ labeling of peptides, proteins and nucleotides. |
| Synonyms | 5-FAM LPETGG, 5-FAM-LPETGG, 5-FAM-LPETGG-OH, FP-020, FP020, fluorescent label, sortase |
| Molecular Weight | 930.83 Da |
| Target | Protein-sorting transpeptidase |
| Amino Acid Sequence | 5-FAM-LPETGG |
| Form | Freeze dried solid |
| Purity | >95% by HPLC |
| Storage | Store desiccated, frozen and in the dark |
| Notes | Modifications: 5-FAM at N terminal |
| References |
Tanaka et al (2008) Site-specific protein modification on living cells catalyzed by Sortase. Chembiochem. 9(5) 802 PMID: 18297670Popp and Ploegh (2011) Making and Breaking Peptide Bonds: Protein Engineering Using Sortase. Angew. Chem. Int. Ed. 50 5024 PMID: 21538739Theile et al (2013) Site-specific N-terminal labeling of proteins using sortase-mediated reactions. Nat. Protoc. 8(9) 1800 PMID: 23989674 Related areasAll protein-sorting transpeptidase modulators >All fluorescent labelled peptides> |
| Supplier | Isca Biochemicals Limited |
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