5-FAM-LPETGG

Category: Bioreagents
Catalog
FP-020
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Product Name 5-FAM-LPETGG
Description 5-FAM-LPETGG is the sortase recognition sequence, LPETGG, coupled to the fluorescent label 5-FAM. 5-FAM-LPETGG can be used for site-specific labeling of target proteins using sortase-catalyzed transpeptidation. In this process, sortase A cleaves between the thr and gly residues, forming a thioester intermediate with the peptide probe. A protein N-terminally engineered with a series of glycine residues resolves this intermediate, regenerating the active site cysteine of sortase and conjugating the probe to the N-terminus of the protein. 5-FAM-LPETGG contains the a 5- carboxyfluorescein label, which is one of the most widely used green fluorescent reagents for in situ labeling of peptides, proteins and nucleotides.
Synonyms 5-FAM LPETGG, 5-FAM-LPETGG, 5-FAM-LPETGG-OH, FP-020, FP020, fluorescent label, sortase
Molecular Weight 930.83 Da
Target Protein-sorting transpeptidase
Amino Acid Sequence 5-FAM-LPETGG
Form Freeze dried solid
Purity >95% by HPLC
Storage Store desiccated, frozen and in the dark
Notes Modifications: 5-FAM at N terminal
References Tanaka et al (2008) Site-specific protein modification on living cells catalyzed by Sortase. Chembiochem. 9(5) 802 PMID: 18297670Popp and Ploegh (2011) Making and Breaking Peptide Bonds: Protein Engineering Using Sortase. Angew. Chem. Int. Ed. 50 5024 PMID: 21538739Theile et al (2013) Site-specific N-terminal labeling of proteins using sortase-mediated reactions. Nat. Protoc. 8(9) 1800 PMID: 23989674
Related areas
All protein-sorting transpeptidase modulators >All fluorescent labelled peptides>
Supplier Isca Biochemicals Limited

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