5-HT ELISA kit (General species)

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CEA808Ge
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Product Name 5-HT ELISA kit (General species)
Description This assay has high sensitivity and excellent specificity for detection of 5-Hydroxytryptamine (5-HT). No significant cross-reactivity or interference between 5-Hydroxytryptamine (5-HT) and analogues was observed.
Synonyms 5HT; Serotonin; N-Methyl-Gamma
Method Competitive
Detection Range 11.11-900ng/mL
Sensitivity 3.97ng/mL
Target 5-HT
Specificity This assay has high sensitivity and excellent specificity for detection of 5-Hydroxytryptamine (5-HT). No significant cross-reactivity or interference between 5-Hydroxytryptamine (5-HT) and analogues was observed.
Sample Types Serum And Plasma
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately.
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Ginsenoside rich fraction of Panax ginseng C.A. Meyer improve feeding behavior following radiation-induced pica in rats;Sensitization induces hypersensitivity in trigeminal nerve;Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats;Catha edulis chewing effects on treatment of paranoid schizophrenic patients;Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression;Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPE;Metabolic Features of Heart Failure with Different Etiology ;Valproate Attenuates Nitroglycerin-Induced Trigeminovascular Activation by Preserving Mitochondrial Function in a Rat Model of Migraine;PI3K/AKT signaling pathway activation in a rat model of migraine.;The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic …;Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids;Changes in sexual performance and biochemical characterisation of functional neural regions: A study in serotonin transporter knockout male rats;Serotonin-Mediated Cardiac Analgesia Via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic Infarction;TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway Inflammation;Neurotransmitter system aberrations in patients with drug addiction;5-HT ;Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome;Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡­;O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress;Investigation of the effect of training on serotonin, melatonin and hematologic parameters in adolescent basketball players;Correlation between intestinal microbiotal imbalance and 5‐HT metabolism, immune inflammation in chronic unpredictable mild stress male rats;
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