| Product Name | AAV1 standard material (eGFP) |
|---|---|
| Description |
Our AAV1 standard material consists of fully assembled AAV1 capsids with an eGFP reporter gene controlled by a CMV promotor. Final concentrations (total capsids and viral genome titer) are lot-specific and can be found on the CoA. The AAV1 standard material is provided with a titer above 1.0E+12 viral genomes/ml in a liquid formulation. Since the buffer does not contain any stabilizing proteins or dyes, the capsids can be used in various applications, including dot blot, western blot, ELISA, qPCR, ddPCR and cell-based assays. Our material contains only a very low remaining amount of iodixanol allowing DLS-SLS-UV/Vis analysis. The lot-specific titers were assigned using AAV1 ELISA (PRAAV1) according to our internally established standard material* and PCR using eGFP primers. Our comprehensive quality control ensures well-characterized capsid material, which can be implemented as reference material in a variety of assays to prove the validity of the corresponding assay. PROGEN provides eGFP-filled AAV standard material for the AAV serotypes 1, 2, 3, 5, 6, 8, 9, rh10, and rh74. *Our internal standard material for each serotype was characterized according to the protocol described in our poster Developing Reliable AAV Standards for ELISA (available in the downloads tab). Data on the establishment of standard material for specific serotypes can be found as part of the performance data for the corresponding ELISAs or can be provided upon request. |
| Source | Produced in HEK293T cells |
| Applications | ELISA, DOT, WB |
| Form | PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl |
| Concentration | > 1.0E+12 vg/ml; please find the lot-specific concentration (total capsid and viral genome titer) on the CoA |
| Purity | > 95% by SDS-PAGE and silver stain |
| Storage | Up to 2 weeks: 2-8°C; long term storage in aliquots at -80°C; avoid > 5 freeze/thaw cycles |
| Notes | Please centrifuge before opening to ensure complete recovery of vial contents; aliquoting and repeated freeze/thaw cycles can lead to a drop in titer |
| Supplier | Progen |
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