| Product Name | AAV5 empty capsids |
|---|---|
| Description |
Our AAV5 empty capsids consist of fully assembled, empty AAV5 capsids and have a titer of > 5.0E+12 viral particles/ml (vp/ml). Final concentrations are lot-specific and can be found on the corresponding vial. The AAV5 empty capsids are provided with titers above 5.0E+12 viral particles/ml in a liquid formulation. Since the buffer does not contain any stabilizing proteins or dyes, the capsids can be used in various applications, including dot blot, western blot and ELISA. The lot-specific titers were assigned according to our internally established standard material* using two different AAV5 ELISA (PRAAV5 and PRAAV5XP). Our comprehensive quality control ensures well-characterized capsid material which can be implemented as reference material in a variety of assays to prove the validity of the corresponding assay.PROGEN also provides empty capsid material for the AAV serotypes 1, 2, 6, 8 and 9.*Our internal standard material for each serotype was characterized according to the protocol described in our poster „Developing Reliable AAV Standards for ELISA“ (available in the downloads tab). Data on the establishment of standard material for specific serotypes can be found as part of the performance data for the corresponding ELISAs or can be provided upon request. |
| Source | Produced in HEK293T cells |
| Applications | ELISA, DOT, WB |
| Form | PBS + 0.014% Tween20 + 1 mM MgCl2 + 2.5 mM KCl |
| Concentration | > 5.0E+12 capsids/ml; please find the lot-specific concentration on the CoA and on the vial |
| Purity | > 95% by SDS-PAGE and silver stain |
| Storage | Up to 2 weeks: 2-8°C; long term storage in aliquots at -80°C; avoid > 5 freeze/thaw cycles |
| Notes | Please centrifuge before opening to ensure complete recovery of vial contents; repeated freeze/thaw cycles and aliquoting can lead to a drop in titer |
| Supplier | Progen |
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