| Product Name | AAVrh74 ELISA Control |
|---|---|
| Description | The AAVrh74 ELISA Control consists of fully assembled, empty AAVrh74 capsids. The concentration is lot specific. The AAVrh74 ELISA Control can be used to generate a standard curve adapting PROGEN's AAVrh10 Titration ELISA (Cat. No. PRAAV10). Two-fold serial dilutions of the AAVrh74 ELISA Control in ASSB 1x buffer (provided with the ELISA kit) result in a typical titration curve. The curve allows the quantitative determination of samples of an unkown particle titer. The AAVrh74 ELISA control can also be used as a positive control with PROGEN's AAVrh10 ELISA. The AAVrh74 ELISA Control has been calibrated on an internally established reference standard. The internal reference standard is a preparation of full AAVrh74 capsids that has been characterized by qPCR and ddPCR (DNA quantification) and electron microscopy (ratio of full to empty capsids). |
| Applications | ELISA |
| Form | Lyophilized, empty AAVrh74 capsids. Reconstitute in 500 µl ASSB 1x (provided with PROGEN's AAV ELISAs), incubate 5 min at RT & mix by rolling 5 min. Avoid vortexing! The final solution contains stabilizing protein, phenol red & ASSB 1x buffer |
| Concentration | 2.6E+09-4.4E+09 capsids/ml after reconstitution in 500 µl ASSB 1x (please find the lot-specific concentration on the CoA and on the vial) |
| Storage | 2-8°C |
| Supplier | Progen |
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