| Product Name | Ac-DEVD-AMC |
|---|---|
| Description | Ac-DEVD-AMC is a fluorogenic substrate for caspase-3 with a Km value of 9.7 ± 1.0 μM. The sequence of Ac-DEVD-AMC is based on the PARP cleavage site Asp216 for caspase-3 and it is also a substrate for caspases -1, -4, -7 and -8. Ac-DEVD-AMC can be used to monitor intracellular caspase activity with cleaved AMC moieties, reflecting caspase activity, being detected by fluorescence. |
| Synonyms | 169332-61-0, Ac-DEVD-AMC, DEVD, PI-260, PI260, caspase-3 , substrate |
| Molecular Weight | 675.7 Da |
| Target | Caspases |
| Amino Acid Sequence | DEVD |
| Form | Lyophilised solid |
| Purity | >97% (HPLC) |
| Cas Number | 169332-61-0 |
| Storage | We recommend storage desiccated, frozen and in the dark |
| Notes | Modifications: Acetyl at N terminal, 7-amino-4-methylcoumarin (AMC) at C terminal |
| References |
Nicholson et al (1995) Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 376(6535) 37 PMID: 7596430Alsuwaidi et al (2013) Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol. J. 10 22 PMID: 23320837Alsuwaidi et al (2013) Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes 6 12 PMID: 23311890 Related areasAll caspase modulators >All fluorescent labelled peptides >All substrate peptides > |
| Supplier | Isca Biochemicals Limited |
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