| Product Name | ACE2 antibody (MSVA-919R) |
|---|---|
| Description | ACE2 antibody validated for immunohistochemistry on 76 different Normal Tissues |
| Synonyms | Angiotensin I converting enzyme 2 |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | MSVA-919R |
| Conjugate | Unconjugated |
| Immunogen | Recombinant fragment (around aa705-805) of human ACE2 (exact sequence is proprietary) |
| Isotype | IgG |
| Reactivity | Human |
| Applications | IHC |
| Working Dilutions | 100-200 |
| Uniprot | Q9BYF1 |
| Storage | Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required. |
| Notes | Positive Control: Kidney: Cells of proximal tubuli should show a moderate to strong ACE2 immunostaining with accentuation of the staining at the apical membrane.. Negative Control: Kidney: Cells of distal tubuli and glomeruli should not show ACE2 immunostaining.. Cellular Localization: Cytoplasmic. Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. |
| Supplier | MSVA |
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