ACH ELISA kit (General species)

Category: Assay Kits
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CEA912Ge
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Product Name ACH ELISA kit (General species)
Description This assay has high sensitivity and excellent specificity for detection of Acetylcholine (ACH). No significant cross-reactivity or interference between Acetylcholine (ACH) and analogues was observed.
Method Competitive
Detection Range 12.35-1,000pg/mL
Sensitivity 5.31pg/mL
Target ACH
Specificity This assay has high sensitivity and excellent specificity for detection of Acetylcholine (ACH). No significant cross-reactivity or interference between Acetylcholine (ACH) and analogues was observed.
Sample Types Serum, Plasma And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately.
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
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