ACTB CLIA kit (Human/Mouse/Rat)

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Product Name ACTB CLIA kit (Human/Mouse/Rat)
Description This assay has high sensitivity and excellent specificity for detection of Actin Beta (ACTb). No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed.
Synonyms Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
Method Sandwich
Detection Range 0.14-100ng/mL
Sensitivity 0.06ng/mL
Target ACTB
Specificity This assay has high sensitivity and excellent specificity for detection of Beta Actin (ACTB). No significant cross-reactivity or interference between Beta Actin (ACTB) and analogues was observed.
Reactivity Human, Mouse, Rat
Sample Types Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 100uL Substrate Solution.Incubate 10 minutes at 37℃; 8.Read RLU value immediately.
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Chemiluminescence
Supplier Cloud Clone

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