| Product Name | ACTH ELISA Kit |
|---|---|
| Description | ACTH ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of ACTH in serum, plasma, tissue homogenates, and other biological fluids. |
| Method | Sandwich |
| Detection Range | 12.5-800 pg/ml |
| Sensitivity | 9.375 pg/ml |
| Target | ACTH |
| Reactivity | All |
| Applications | ELISA |
| Sample Types | Serum, plasma, tissue homogenates, and other biological fluids. |
| Reconstitution | ACTH ELISA Kit (A86990) employs the sandwich enzyme immunoassay technique for the quantitative measurement of universal ACTH in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for ACTH has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the ACTH present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-ACTH Antibody, which binds the captured ACTH present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of ACTH captured in each well. The concentration of ACTH can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve. |
| Storage | Store at +4°C. Do not use past expiration date! |
| Supplier | Antibodies.com |
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