| Product Name | ACTH ELISA kit (Mouse) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH). No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed. |
| Synonyms | Corticotropin; Adrenocorticotropin |
| Method | Competitive |
| Detection Range | 12.35-1,000pg/mL |
| Sensitivity | 4.94pg/mL |
| Target | ACTH |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH). No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed. |
| Reactivity | Mouse |
| Sample Types | Serum, Plasma And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately. |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Ursolic acid suppresses growth and adrenocorticotrophic hormone secretion in AtT20 cells as a potential agent targeting adrenocorticotrophic hormone-producing pituitary adenoma.;High activity of the stress promoter contributes to susceptibility to stress in the tree shrew;Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus.;;Triptolide suppresses growth and hormone secretion in murine pituitary corticotroph tumor cells via NF-kappaB signaling pathway;Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary …;Metformin suppresses growth and adrenocorticotrophic hormone secretion in mouse pituitary corticotroph tumor AtT20 cells;Chronic hippocampal abnormalities and blunted HPA axis in an animal model of repeated unpredictable stress;Comparison between men and women of volume regulating hormones and aquaporin-2 excretion following graded central hypovolemia;Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive-and anxiety-like behaviour, and gut microbiome composition;Hypothalamus-pituitary-adrenal axis imbalance and inflammation contribute to sex differences in separation-and restraint-induced depression;Repeated unpredictable stress and social isolation induce chronic HPA axis dysfunction and persistent abnormal fear memory;Altered glucocorticoid metabolism represents a feature of macroph‐aging;Exposure to eagle owl feces induces anti-predator behavior, physiology, and hypothalamic gene responses in a subterranean rodent, the plateau zokor (Eospalax …; |
| Supplier | Cloud Clone |
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