ADPN ELISA kit (Human)

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Product Name ADPN ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Adiponectin (ADP). No significant cross-reactivity or interference between Adiponectin (ADP) and analogues was observed.
Synonyms GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
Method Sandwich
Detection Range 0.156-10ng/mL
Sensitivity 0.061ng/mL
Target ADPN
Specificity This assay has high sensitivity and excellent specificity for detection of Adiponectin (ADPN). No significant cross-reactivity or interference between Adiponectin (ADPN) and analogues was observed.
Reactivity Human
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot Q15848
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Relation between afamin level and some inflammatory markers in obese children;Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case- …;Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case-Control Study;Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD?;Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in …;Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity;Relationship between obesity and iron deficiency anemia: is there a role of hepcidin?;Adiponectin may be used as a marker in prediction of diabetic retinopathy patients;Combinational Effect of Pine Needle Polysaccharide and Kudzu Flavonoids on Cell Differentiation and Fat Metabolism in 3T3-L1 Cells;Effect of fermented food consumption on biochemical parameters and adipokines levels;Expression of adipokines and their receptors in adipose tissue of women with class 3 obesity with or without hypertension;High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …;Increased plasma osteopontin levels are associated with nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus;Serum Asprosin Concentrations Are Increased and Associated with Insulin Resistance in Children with Obesity;The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children;HOMA-Adiponectin Closely Associates with Cardiometabolic Risk Markers in Middle-Aged Indians with Metabolic Syndrome;Adipocytokines and Metabolic Syndrome in Patients with Schizophrenia;Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity;Serum ZAG and Adiponectin Levels Were Closely Related to Obesity and the Metabolically Abnormal Phenotype in Chinese Population;Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with …;The effect of nutrition and exercise training on irisin and semaphorin-3E levels in obese patients;Exercise-Induced Increases in Insulin Sensitivity After Bariatric surgery are Mediated by Muscle Extracellular Matrix Remodeling;Study of serum adiponectin and resistin in patients with obstructive sleep apnea;Association of+ 10211T/G (Rs17846866) Variant of Adiponectin Gene With Type 2 Diabetes Mellitus;The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren;Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren;Decreased Plasma MANF Levels are Associated with Type 2 Diabetes;Altered Serum Marker of Adipokines Profile in Breast Cancer Women;The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study;GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity;Serum and salivary adipokines in type 2 diabetes–Results of a pilot study in India;Adiponectin Levels Are Associated with White Matter Lesions (WMLs) and Cognitive Impairment;High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients;SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions.;
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