ADPN ELISA kit (Rat)

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Product Name ADPN ELISA kit (Rat)
Description This assay has high sensitivity and excellent specificity for detection of Adiponectin (ADP). No significant cross-reactivity or interference between Adiponectin (ADP) and analogues was observed.
Synonyms GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
Method Sandwich
Detection Range 0.312-20ng/mL
Sensitivity 0.127ng/mL
Target ADPN
Specificity This assay has high sensitivity and excellent specificity for detection of Adiponectin (ADPN). No significant cross-reactivity or interference between Adiponectin (ADPN) and analogues was observed.
Reactivity Rat
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot Q8K3R4
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet;Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese Rats;Perirenal fat associated with microalbuminuria in obese rats;Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats;Perinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar rats;Adipose tissue chromium and vanadium disbalance in high-fat fed Wistar rats;Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats;Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats;Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway;Swimming training attenuates the morphological reorganization of the myocardium and local inflammation in the left ventricle of growing rats with untreated experimental diabetes.;Decreased adipose tissue zinc content is associated with metabolic parameters in high fat fed Wistar rats;Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines;Trigonella foenum-graecum water extract improves insulin sensitivity and stimulates PPAR and γ gene expression in high fructose-fed insulin-resistant rats;Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats;Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats;Central administration of galanin receptor 1 agonist boosted insulin sensitivity in adipose;Berberis integerrima ameliorates insulin resistance in high-fructose-fed insulin-resistant rats;Differential Susceptibility of Germ and Leydig Cells to Cadmium-Mediated Toxicity: Impact on Testis Structure, Adiponectin Levels, and Steroidogenesis;Antihyperglycemic Effect of Rosa Damascena is Mediated by PPAR.γ Gene Expression in Animal Model of Insulin Resistance;Aging effects on circulating adiponectin levels and the expressions of adiponectin and adiponectin receptor 1 in the brains of male rats;Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats;Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome;Interrelation of liver vascularity to non-alcoholic fatty liver through a comparative study of the vasodilator effect of carvedilol or nicorandil in rats;Resemblance and differences in dietary restriction nephroprotective mechanisms in young and old rats;Activation of central galanin receptor 2 mitigated insulin resistance in adipocytes of diabetic rats;Cooperative effects of galanin and leptin on alleviation of insulin resistance in adipose tissue of diabetic rats;Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡­;Activation of the Peroxisome Proliferator-Activated Receptors (PPAR-α/γ) and the Fatty Acid Metabolizing Enzyme Protein CPT1A by Camel Milk Treatment …;Vitamin D3 enhances the effects of omega‐3 oils against metabolic dysfunction‐associated fatty liver disease in rat;Associations of imbalance of intestinal flora with severity of disease, inflammatory factors, adiponectin, and vascular endothelial function of hypertension patients;
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