| Product Name | AF488 NHS ester |
|---|---|
| Description | AF488 is a bright and photostable dye, equivalent of Alexa Fluor® 488. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy. From the chemical standpoint, AF488 is a sulfonated rhodamine dye, Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated though - otherwise, use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-AF488. This NHS ester is an amine reactive dye, meaning it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules. |
| Molecular Weight | 732.74 kDa |
| Form | Dark orange solid. |
| Purity | > 80% (by 1H NMR and HPLC-MS). The balance is mostly carboxylic acid. |
| Storage | Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate. |
| Supplier | Antibodies.com |
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