| Product Name | AFP ELISA kit (Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Alpha-Fetoprotein (aFP). No significant cross-reactivity or interference between Alpha-Fetoprotein (aFP) and analogues was observed. |
| Synonyms | aFP; A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein |
| Method | Sandwich |
| Detection Range | 0.312-20ng/mL |
| Sensitivity | 0.127ng/mL |
| Target | AFP |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Alpha-Fetoprotein (AFP). No significant cross-reactivity or interference between Alpha-Fetoprotein (AFP) and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Serum, Plasma, Tissue Homogenates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P02773 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model;Polyol profile as an early diagnostic and prognostic marker in natural product chemoprevention of hepatocellular carcinoma in diabetic rats;Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver;Cytokines as important playmakers of experimental hepatocarcinogenesis confounded by diabetes.;Chemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar rats;Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme;Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats;Effect of Mesenchymal Stem Cells on Transforming Growth Factor Beta Level in Hepatocellular Carcinoma Induced Rat Model;Antiangiogenic activity of vitexicarpine in experimentally induced hepatocellular carcinoma: Impact on vascular endothelial growth factor pathway;Mesenchymal stem cell therapy of hepatocellular carcinoma in rats: Detection of cell homing and tumor mass by magnetic resonance imaging using iron oxide nanoparticles;Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats;Dynamic Volume Assessment of Hepatocellular Carcinoma in Rat Livers Using a Clinical 3T MRI and Novel Segmentation;Impact of Mesenchymal Stem Cells and Vitamin D on Transforming Growth Factor Beta Signaling Pathway in Hepatocellular Carcinoma in Rats;Chemoprotective Efficacy of Curcumin and Flax Seed Oil in 1, 2-Dimethylhydrazine Induced Rat Colon Cancer;Ameliorative effects of honey and venom of honey bee on induced colon cancer in male albino rats by1, 2 dimethylhydrazine;Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma;Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats;Fucoidan Ameliorates Hepatocellular Carcinoma Induced in Rats: Effect on miR143 and Inflammation;Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats;Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo;QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model;In Vivo Study of a Newly Synthesized Chromen-4-One Derivative as an Antitumor Agent Against HCC; |
| Supplier | Cloud Clone |
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