| Product Name | AGR2 ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Anterior Gradient Protein 2 (AGR2). No significant cross-reactivity or interference between Anterior Gradient Protein 2 (AGR2) and analogues was observed. |
| Synonyms | GOB4; HAG2; XAG2; AG2; PDIA17; HPC8; Anterior Gradient Protein 2; Protein Disulfide Isomerase Family A,Member 17; Secreted cement gland protein XAG-2 homolog |
| Method | Sandwich |
| Detection Range | 15.6-1,000pg/mL |
| Sensitivity | 6.0pg/mL |
| Target | AGR2 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Anterior Gradient 2 (AGR2). No significant cross-reactivity or interference between Anterior Gradient 2 (AGR2) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | O95994 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Serum AGR2 as an early diagnostic and postoperative prognostic biomarker of human lung adenocarcinoma;Integrated Proteomic Profiling of Cell Line Conditioned Media and Pancreatic Juice for the Identification of Pancreatic Cancer Biomarkers;Anterior gradient 2 (AGR2): blood-based biomarker elevated in metastatic prostate cancer associated with the neuroendocrine phenotype;Validation of four candidate pancreatic cancer serological biomarkers that improve the performance of CA19.9;Validation of Biomarkers That Complement CA19.9 in Detecting Early Pancreatic Cancer;AGR3 in Breast Cancer: Prognostic Impact and Suitable Serum-Based Biomarker for Early Cancer Detection;Serum AGR2 as a useful biomarker for pituitary adenomas;Identification, characterization and application of a new peptide against anterior gradient homolog 2 (AGR2);Anterior gradient protein 2 is a marker of tumor aggressiveness in breast cancer and favors chemotherapy‑induced senescence escape;Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancer; |
| Supplier | Cloud Clone |
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