Product Name | Ak1, 1-210aa, Mouse (Bioactivity Validated) |
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Description | Ak1, also known as Adenylate kinase isoenzyme 1 isoform 1, is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes.It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in Ak1 are the cause of a form of hemolytic anemia. Recombinant mouse Ak1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Synonyms | Adenylate kinase Isoenzyme 1 isoform 1, Ak-1, B430205N08Rik |
Host | E. coli |
Molecular Weight |
23.9kDa (224aa) 18-28kDa (SDS-PAGE under reducing conditions) |
Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMGCCVSS EPQEEGGRKT GEKLKKAKII FVVGGPGSGK GTQCEKIVQK YGYTHLSTGD LLRAEVSSGS ERGKKLSAIM EKGELVPLDT VLDMLRDAML AKVDSSNGFL IDGYPREVKQ GEEFEQKIGQ PTLLLYVDAG AETMTQRLLK RGETSGRVDD NEETIKKRLE TYYNATEPVI SFYDKRGIVR KVNAEGTVDT VFSEVCTYLD SLK |
Tag | His-tag |
Reactivity | Mouse |
Applications | SDS-PAGE |
Form | Liquid, in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol. |
Concentration | 0.5mg/ml (determined by Absorbance at 280nm) |
Purity | > 90% by SDS-PAGE |
Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
References | Dzeja PP., et al. (2007) J Biol Chem. 282(43):31366-72. Ravera S., et al., (2015) Curr Eye Res. 32(3):249-57. |
Background | Ak1, also known as Adenylate kinase isoenzyme 1 isoform 1, is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes.It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in Ak1 are the cause of a form of hemolytic anemia. Recombinant mouse Ak1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Supplier | ARP |
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