| Product Name | Alanine Aminotransferase Activity Assay Kit |
|---|---|
| Description | This Alanine Aminotransferase Activity Assay Kit is a quantitative assay kit for measuring Alanine Aminotransferase activity in animal tissues, plant tissues, cell culture (adherent or suspension cells), serum, and bacteria. |
| Method | Quantitative |
| Target | Alanine Aminotransferase |
| Applications | Functional Studies |
| Sample Types | Animal tissues, plant tissues, cell culture (adherent or suspension cells), serum, and bacteria. |
| Reconstitution | This kit provides a simple method for detecting alanine aminotransferase (ALT) activity in a variety of biological samples such as serum, plasma, animal and plant tissues, cells, and bacteria. In the assay, ALT catalyzes the transamination reaction of alpha-ketoglutarate and alanine at 37°C and pH7.4 to produce glutamic acid and pyruvate; pyruvate can react with 2,4-dinitrophenylhydrazine to form pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm reflects ALT activity. |
| Storage | Shipped at 4°C. Store at 4°C in the dark. Do not use past expiration date! |
| Supplier | Antibodies.com |
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