Product Name | ALD ELISA kit (General species) |
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Description | This assay has high sensitivity and excellent specificity for detection of Aldosterone (ALD). No significant cross-reactivity or interference between Aldosterone (ALD) and analogues was observed. |
Method | Competitive |
Detection Range | 24.69-2,000pg/mL |
Sensitivity | 9.41pg/mL |
Target | ALD |
Specificity | This assay has high sensitivity and excellent specificity for detection of Aldosterone (ALD). No significant cross-reactivity or interference between Aldosterone (ALD) and analogues was observed. |
Sample Types | Serum, Plasma And Other Biological Fluids |
Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately. |
Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
Notes | Colormetric |
References | High Salt Intake Down-Regulates Colonic Mineralocorticoid Receptors, Epithelial Sodium Channels and 11β-Hydroxysteroid Dehydrogenase Type 2;Evidence for glucocorticoid&-mediated hypertension after uninephrectomy;Relevance of a Hypersaline Sodium-Rich Naturally Sparkling Mineral Water to the Protection against Metabolic Syndrome Induction in Fructose-Fed Sprague-Dawley Rats: A Biochemical, Metabolic, and Redox Approach;Relevance of a Hypersaline Sodium-Rich Naturally Sparkling Mineral Water to the Protection against Metabolic Syndrome Induction in Fructose-Fed Sprague-Dawley Rats: A Biochemical, Metabolic, and Redox Approach.;Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder;H,K-ATPase type 2 contributes to salt-sensitive hypertension induced by K(+) restriction.;Magnitude of Resistant Hypertension and Impact of Aldosterone to Renin Ratio In Resistant Hypertension;Effects of renal denervation on monocrotaline induced pulmonary remodeling;Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy;Regeneration of Functional Adrenal Tissue Following Bilateral Adrenalectomy.;Acute adrenal cortex injury during cardiopulmonary bypass in a canine model;ANP-stimulated sodium secretion in the collecting duct prevents sodium retention in renal adaptation to acid load;MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model;Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats;Preventive preclinical efficacy of intravenously administered sphingosine-1-phosphate (S1P) in strengthening hypoxia adaptive responses to acute and sub-chronic …;The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice;Decreased KLHL3 expression is involved in the activation of WNK-OSR1/SPAK-NCC cascade in type 1 diabetic mice; |
Supplier | Cloud Clone |
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