Product Name | ALP ELISA kit (Rat) |
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Description | This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase (ALP). No significant cross-reactivity or interference between Alkaline Phosphatase (ALP) and analogues was observed. |
Synonyms | AKP; ALKP; Basic Phosphatase |
Method | Sandwich |
Detection Range | 1.25-80ng/mL |
Sensitivity | 0.53ng/mL |
Target | ALP |
Specificity | This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase (ALP). No significant cross-reactivity or interference between Alkaline Phosphatase (ALP) and analogues was observed. |
Reactivity | Rat |
Sample Types | Serum, Plasma, Tissue Homogenates And Other Biological Fluids |
Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
Notes | Colormetric |
References | The role of atorvastatin in bone metabolism in male albino Wistar rats;The active role of osteoporosis in the interaction between osteoblasts and bone metastases;A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration;Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats.;Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway.;Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats;Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats;The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats;The decline of whole-body glucose metabolism in ovariectomized rats;Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents;Osteoprotective Activity and Metabolite Fingerprint via UPLC/MS and GC/MS of Lepidium sativum in Ovariectomized Rats;МАРКЕРЫ КОСТНОГО РЕМОДЕЛИРОВАНИЯ ПРИ ЗАМЕЩЕНИИ ДЕФЕКТАТРАБЕКУЛЯРНОЙ КОСТНОЙ ТКАНИ РЕЗОРБИРУЕМЫМИ И …;Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing; |
Supplier | Cloud Clone |
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