| Product Name | ALPL ELISA kit (Mouse) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase, Liver/Bone/Kidney (ALPL). No significant cross-reactivity or interference between Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) and analogues was observed. |
| Synonyms | BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase,Tissue-Nonspecific Isozyme; Alkaline Phosphatase, Liver/Bone/Kidney |
| Method | Sandwich |
| Detection Range | 0.156-10ng/mL |
| Sensitivity | 0.058ng/mL |
| Target | ALPL |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase, Tissue-nonspecific (ALPL). No significant cross-reactivity or interference between Alkaline Phosphatase, Tissue-nonspecific (ALPL) and analogues was observed. |
| Reactivity | Mouse |
| Sample Types | Serum, Plasma, Tissue Homogenates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P09242 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-κB activation;Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice;Enhanced differentiation of osteoblastic cells on novel chitosan/β-1, 3-glucan/bioceramic scaffolds for bone tissue regeneration;New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization.;MiR-142-5p promotes bone repair by maintaining osteoblast activity;New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological …;MiR‑142‑5p promotes bone repair by maintaining osteoblast activity;Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In Vivo;Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice;Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9;The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro; |
| Supplier | Cloud Clone |
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