| Product Name | ALT ELISA kit (Mouse) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Alanine Aminotransferase (ALT). No significant cross-reactivity or interference between Alanine Aminotransferase (ALT) and analogues was observed. |
| Synonyms | GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1 |
| Method | Sandwich |
| Detection Range | 1.56-100ng/mL |
| Sensitivity | 0.61ng/mL |
| Target | ALT |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Alanine Aminotransferase (ALT). No significant cross-reactivity or interference between Alanine Aminotransferase (ALT) and analogues was observed. |
| Reactivity | Mouse |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | Q8QZR5 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Enhanced antitumor activity by combining an adenovirus harboring ING4 with cisplatin for hepatocarcinoma cells;Additive effects of nicotine and high-fat diet on hepatic steatosis in male mice.;Pathogenesis of emerging severe fever with thrombocytopenia syndrome virus in C57/BL6 mouse model;Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanisms;Bifidobacterium pseudocatenulatum CECT7765 promotes a TLR2-dependent anti-inflammatory response in intestinal lymphocytes from mice with cirrhosis;Resolvin E1 and chemerin C15 peptide do not improve rodent non-alcoholic steatohepatitis;Dihydromyricetin protects against liver ischemia/reperfusion induced apoptosis via activation ofFOXO3a-mediated autophagy.;Alcohol-associated intestinal dysbiosis impairs pulmonary host defense against Klebsiella pneumoniae.;A critical role of hepatitis B virus polymerase in cirrhosis, hepatocellular carcinoma, and steatosis;Brg1 promotes liver regeneration after partial hepatectomy via regulation of cell cycle;Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy;CD47 Deficiency in Mice Exacerbates Chronic Fatty Diet-Induced Steatohepatitis Through Its Role in Regulating Hepatic Inflammation and Lipid Metabolism;Taraxasterol mitigates Con A-induced hepatitis in mice by suppressing interleukin-2 expression and its signaling in T lymphocytes;Blockade of mIL‐6R alleviated lipopolysaccharide‐induced systemic inflammatory response syndrome by suppressing NF‐κB‐mediated Ccl2 expression and …;Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activation; |
| Supplier | Cloud Clone |
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