| Product Name | ALT ELISA kit (Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Alanine Aminotransferase (ALT). No significant cross-reactivity or interference between Alanine Aminotransferase (ALT) and analogues was observed. |
| Synonyms | GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1 |
| Method | Sandwich |
| Detection Range | 0.78-50ng/mL |
| Sensitivity | 0.31ng/mL |
| Target | ALT |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Alanine Aminotransferase (ALT). No significant cross-reactivity or interference between Alanine Aminotransferase (ALT) and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P25409 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and -independent mechanisms;Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in rats;Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in rats;Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning;The effects of subacute deltamethrin exposure on rat liver: histochemical, immunohistochemical, and biochemical study;Advanced Studies in Clinical and Experimental Research in Gastroenterology;Effects of developmental exposure to silver in ionic and nanoparticle form: A study in rats;Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway.;Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats;Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury Model;The valuable effects of potent antioxidant curcumin in cisplatin induced liver and kidney injury;Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents;Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet;Biological Safety and Biodistribution of Chitosan Nanoparticles;Effects of Tempol in Lipopolysaccharide-Induced Liver Injury;An Experimental Study: Benefits of Digoxin on Hepatotoxicity Induced by Methotrexate Treatment;Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver …; |
| Supplier | Cloud Clone |
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