| Product Name | Anti-Adiponectin Antibody [5H7] |
|---|---|
| Description | Mouse monoclonal [5H7] antibody to Adiponectin. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5H7 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant human adiponectin (amino acids 15-244) purified from E. coli . |
| Isotype | IgG1 |
| Molecular Weight | Approximately 64 kDa in mouse liver cell lysates. |
| Target | Adiponectin |
| Amino Acid Sequence | GHDQETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN |
| Protein Length | 230 |
| Specificity | This antibody recognises the collagen-like domain of human adiponectin, also known as Acrp30, Gelatin-binding protein, Adipose most abundant gene transcript 1 protein, Adipocyte complement-related 30 kDa protein or apM-1. Adiponectin a 244 amino acid ~30 kDa major adipokine secreted into the bloodstream from adipose tissue into the circulation where it exists as three distinct, low, medium and high molecular weight oligomeric forms (UniProt: Q15848).Circulating adiponectin levels are correlated with insulin sensitivity and decreased levels parallel the change in insulin sensitivity during the progression to type II diabetes. Mutations in the adiponectin gene can also lead to adiponectin deficiency characterized by obesity, diabetes, high blood pressure and circulating cholesterol. Mouse anti Human adiponectin, clone 5H7 had been used successfully as a detection reagent in the development of sensitive sandwich ELISA's in conjunction with Mouse anti Human adiponectin, clone Adn27 (MCA4652) or Mouse anti Human adiponectin antibody, clone Adn36 (MCA4653) as capture reagents. |
| Reactivity | Human, Mouse |
| Applications | ELISA, WB |
| Working Dilutions | WB: 1:1,000 - 1:2,000 |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 10% Glycerol and 0.02% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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