| Product Name | Anti-ALK Antibody [ALK/1031] |
|---|---|
| Description | Mouse monoclonal [ALK/1031] antibody to ALK. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ALK/1031 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant fragment, around amino acids 400-500, of human ALK protein. The exact sequence is proprietary. |
| Isotype | IgG2b |
| Molecular Weight | 80 kDa (hybrid) / 200 kDa (wild type) |
| Target | ALK |
| Specificity | The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene,NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control. |
| Reactivity | Human |
| Applications | WB |
| Working Dilutions | WB: 1-2 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide. |
| Concentration | 200 µg/ml |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Supplier | Antibodies.com |
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