| Product Name | Anti-alpha Tubulin Antibody [YL1/2] (HRP) |
|---|---|
| Description | Rat monoclonal [YL1/2] antibody to alpha Tubulin (HRP). |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | YL1/2 |
| Conjugate | HRP |
| Immunogen | Yeast tubulin. |
| Isotype | IgG2a |
| Target | alpha Tubulin |
| Specificity | This antibody recognises the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987). Rat anti tubulin alpha antibody, clone YL1/2 is routinely tested in ELISA on tubulin. |
| Reactivity | Jellyfish, Amphibian, Other, Monkey, Xenopus, Aves, Fungi, Echinoderm, Bovine, Saccharomyces, Canine, Drosophila, Human, Insect, Mouse, Plant, Porcine, Rat, Yeast |
| Applications | IHC-Fr, ELISA, WB |
| Working Dilutions | WB: 1:100 - 1:1,000, MCA77P is suitable for use as a loading control. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.01% Thiomersal and HRP Stabiliser (Proprietary stabiliser based on a TRIS buffer, <15ppm CMIT/MIT). |
| Concentration | 1 mg/ml |
| Storage | Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use. |
| Supplier | Antibodies.com |
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